Process of controlling microbial activity



United States Patent "ice 3,285,812 PROCESS OF CONTROLLING MICROBIAL ACTIVITY Robert R. Mod, Evald L. Skau, Sara P. Fore, and Frank C. Magne, New Orleans, Arthur F. Novak, Baton Rouge, and Harold P. Dupuy, Jesse R. Ortega, and Mary J. Fisher, New Orleans, La., assignors to the United States of America as represented by the Secretary of Agriculture No Drawing. Continuation of application Ser. No. 353,012, Nov. 4, 1963. This application June 8, 1965, Ser. No. 462,456

6 Claims. (Cl. 167-63) This application is a continuation of Serial No. 353,012, filed November 4, 1963, as a division of Serial No. 197,- 545, filed May 24, 1962, now US. Patent No. 3,170,265.

A non-exclusive, irrevocable, royalty-free license in the invention hereindescribed, throughout the world for. all purposes of the United States Government, with the power to grant sublicenses for such purposes, is hereby granted to the Government of the United States of America.

This invention relates to certain new nitrogen containing organic compounds. More particularly, this invention relates to derivatives of aliphatic acid amides which exhibit anti-microbial activity. The substituted amides that are the subject of this invention are characterized by the fact that as growth inhibitors, they are effective against a variety of micro-organisms that include bacteria, yeasts, and molds, many of which are pathogenic.

The findings herein disclosed are considered remarkable in that in some notable instances compounds that are closely related from the point of view of chemical architecture exhibit quite opposite effects against the same organisms. For example, one compound may exhibit properties as a growth inhibitor against one particular organism while a closely related counterpart may serve to promote increased growth for the same organism. Some of these amides exhibit broad antimicrobial spectrum, whereas others exhibit selective antimicrobial spectrum.

The compounds which are the subject of this invention are:

4- fi-hydroxycaproyl) morpholine HOCH:(CH:)4CON /O CHr-CH,

N-butyldecanamide CH (CH CONH(CH CH N-isoamyldecanamide CH (CH CONHCH CH CH(CH 2 N-cyclohexyldecanamlde CH;(CHz)aCONHCH OHr-C Q N, N-his (2-decanoyloxyethyl) decanamide CH (CH CON [CH CH OOC(CH CH 3,285,812 Patented Nov. 15, 1966 CH3(CH1)BC ON CH3(CH2):G ON

CHAOHMC ON 4-(6 (7) -hydroxystear0yl) morpholine C Hg C H:

OHflOHfln-wCHOH(CHz)4-5O ON C r-C 4 0le0yl-2,fi-dimethylmorpholine CHr- C H C H s(C 2)7OH: CH(C z)1C ON 0112- 0% C H] 4(12-pr0pionoxyoleoyl) morphollne 0 Hr C CH3(CH2)5CHOH2CH:CH(CH:|)1CON O OOCCHrCH; OHr-O:

lN-tert-butyloleamide CH (CH qCH CH(CH CONHC (CH 3 N-oleoylhexamethylen-lmine C H2C H2 CH:C H: N oleoyl-N-methylpiperazine UH -CH1 N CH;

C Hz-CH, 4-petroselinoylmorphollne GEE-43H;

GET-C14;

MORPHOLIDES OF PARSLEY SEED OIL FATTY ACIDS The new nitrogen-containing compounds which are the subject of this invention were prepared by conventional methods.

extender be inert with respect to the amide involved.

Petroleum jellies, various alcohols and polyols, vegetable oils and the like are suitable.

In the case of intended use as fungicide in the protective coating composition art, the compounds that are the subject of this invention are compatible with conventional and with drying oil modified alkyds, for example.

Specific examples showing the preparation of each of the new compounds being claimed are set forth below along with appropriate data in tabular form which is being submitted for the purpose of establishing the growth inhibiting properties of the claimed compounds.

EXPERIMENTAL Difco Bacto Dehydrated Stock Culture Agar at pH 7.0, Difco Bacto Dehydrated Yeast Morphology Agar at pH 4.5, and Difco Dehydrated Mycological Agar at pH 7.0 were selected to test the inhibition of the bacteria, yeast, and mold cultures, respectively.

Fatty acid derivatives were screened for their antimicrobial activity against two bacteriaa gram positive, Micrococcus pyogenes, and a gram negative, Escherichia coli; several yeasts-Saccharomyces ccrcvisiae, Candida stellatoidea, and Torulopsis sp.; and several molds- Neurospora sp., Alternaria sp., Mucor sp., Hormodendrum sp., Geotrichum sp., Penicillium sp., and Aspergillus sp., as illustrated in Table I. Fatty acid derivatives were screened for their antimicrobial activity against two bacteriaa gram negative, Escherichia coli, and a gram positive, Micrococcus pyogcnes; and many pathogenic molds Candida albicans, Candida wcrnecki, Epidcrmophyton floccosum, Keratinomyccs ajelloi, Microsporum canis, Microsporum cookii, Microsporum gypseum, Microsporum Nanum, T richophyton concentricum, Trichophyton epilans, T richophyzon equinum, Trz'chophyton fcrrugincum, T richophyton gallinae, T richophyton megnini, T richophyton mcntagrophytcs var. intcrdigitales, Trichophyton mentagroplzytes var. granulare, T richophyton rzibrum, Trichoplzyton Sabouraudi, T richophyton schoenlcini, T richophyton sulfurium, Trichophyton tonsurans, and T richophyton violaceum, as illustrated in Tables HA and IIB.

Seeded agar plates were used to measure the antimicrobial activity against bacteria and yeasts. The filter paper disc method was used to evaluate the liquid compounds, and the cylinder plate method was used to evaluate the solid compounds. Standard-size paper discs or uniform-size stainless steel cylinders were placed on the surface of the previously inoculated plates. The liquid compounds were pipetted onto the discs, and the solid com pounds were introduced into the stainless steel cylinders.

Streaked and poured agar plates were used to measure the antimycotic activity against molds. The streaked agar plates were prepared by streaking the hardened agar plates with the test mold, and the poured agar plates were prepared by pouring dillutions of mold spores over the hardened agar plates. The compounds were then added onto specified areas of these streaked and poured agar plates. The paper disc method was used to evaluate the liquid and solid compounds, the solid compounds were simply dissolved in glycerol or ethanol before applying onto the paper disc. The solid compounds were also tested in pure form by introducing the solid material directly on the surface of the inoculated agar plates.

To eliminate any error which could result from an in suflicient number of tests, a minimum of three experiments employing duplicate plates was used for measuring the antimicrobial activity of each compound.

All test plates were incubated at the optimum growing temperature for each organism. The tabulated results illustrated in Table I were obtained from periodic read ings after 1, 2, 3, and 5 days, respectively. The tabulated results illustrated in Tables HA and IIB were obtained from periodic readings after 3, 5, and 7 days, respectively. Zones of inhibition were compared with those of the controls.

TABLE I.THE ANTIMICROBIAL ACTIVITY OF SOME RICINOLEIC AND OLEIC ACID DERIVATIVES Antimicrobial activity Compound Micro-organisms e F G H a The new antimicrobial compound is italicized.

b ++=Good; +=Fair; None; oo=Organism failed to grow over compound; o=Very slight growth over the compound.

0 A=Micmcoccus pyogenes; B=Escherichia colt; C=Saccharomz1ces l++l+lll III II ++1 1+1+1+++1 1 1 1++1 1 1 1 1 1 1 1 1+1 1 1 1 1 1++1 1 +1 1 1 1 1++1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1++1 1 1 1 1 1 1 o 1 1 1 1 1 1 1 o o 1 0 I 1 1 1 1 1 1++1+1 1 1+1+1 1++1+1+++1+++1 1++1+1 1 1 1 1 1 1 1 1 1 1 1 1 1 o 1 1 1+1 1 1+ v ++11 1 =1 1 1 1 1 1++1 1 1 1+1 1 1++1 =1 1 11 1 1 1 1 1 1 1 1 1 1 1+1+ 1++1+1 1 1+1 1 1 1 1 1 1 1 1++1 1 1++1 1+ 1 1+1+ 1 11+1 1 1 1 1 1 1 1 1 10000 i 1++1+11 1 1 =+1 1 i 'i cerevisiae; D Candida .stellatoidea; E .=Torulopsis sp.; F =Neurospora sp.; G=A1ternaria sp.; H=Mucor sp.; I I-Iomodendrum sp.; J Geotrichum sp.; K Pcnicillium sp.; L Aspergillus sp.

TABLE IIA.ANTIMI'CROBIAL ACTIVITY OF SOME FATTY ACID DERIVATIVES Antimicrobial activity h Compound 1 G-hydroxycaproic acid- Microorganisms I 4-(6-hydr01ycaproyl) mor, I 4(6-hydroxycaproyl)-2, fi-dimethylmorpholine Lactonc oi fi-hydroxy-caproic acid Ethyl fi-hydroxycaproate Sodium o-hydroxycaprnafc fi-arninocaproic acid- Laotam 0i 6-aminocaproic acid Nonanoic acid 4-n0nanoylmorpholine -i Decanoic acid 4-decanoylmorpholine 441mmouZ-2,6-dimethyimorpholine IT L 4 1i '4 N 1.: N mflnh'n- H N,N-bia (z-dccanoyloroethul) N-decanoylpiperidine 4 vifl'rn 'nnl 1 a N-decanoyl-Nmethz lpipemzim- N,N-didecanaylpipcmzinzr N-decanoyltetrahydroquinol Laurie acid 4-1auroylmorpholine N ,N-bis (Z-hydrozyethyl)-Zauramide N,N-bia (z-decanolylozyethz l) lama-1m N,N-b1'a (S-Zaumulozyethyl) lauramide Palmitio acid -Palmitoylrnorp oline ..o Stearic acid 'i'stearoylmorp ne 6(7)-hydroxystearic acid i-6(7)-hudroa:7stearqg l) morp 6,7-epoxystearic acid lz-hydroxystearic acid.

4 12-hydroxystcaroyl) morpholine.

Methyl iZ-hydrozystearate #(cpoxystearoyl) morpnoline N,N-bis(Z-acetoxyethyl)epoxystearamide N-N-bls(Z-acetoxyethyl)dlepoxystearamide Oleic acid i-oleoylmorpholine 4-alsoyl-2,6-dimethy1m0rpholine i-(epoxyoctadecenoyl) morpholine Ricinoleic acid 4-ricinolcoylmorphol1ne Methyl ricinoleate.

4-(12-pr0pionozyoleoyl) morpholine N-tert-butyloleamide N,N-bis(2-hydroxyethyl)oleamide N ,N-bis (ii-acetoxyethyDoleamlde N ,Nbis (2-benzolyoxyethy1)oleamide N N-bis 2acetoxyethy1)epoxyoctadecenamide.

-oleo1 1hezamethylenimine..- N-oleoyl-Nmethulpiperazine. Petroselinic acid.

4-1)etroselinoylmorpholine Methyl petroselinate.

Elaidic acid... 4-elaidoylniornhnlim Linoleic acid 4-linoleoylrnorpholine- Dimerized i-linoleoylmorpholine.

Dimerized linoleic acid {Em i 1022 Partially hydrogenated cottonseed acids Morpholides of partially hydrogenated cottonseed acids Morpholides oi partially epoxidized cottonseed acids I N,N-bis(2-acetoxyethyl)amides oi partially epoxidized cottonseed acids 1 Nflqfiois iz-acetoxyethyl)amides oi partially hydrogenated cottonseed ac s Parsley seed acids Morpholz'dea of parcel]! seed oil fatty acids Morpholides oi rapeseed acids N,N-bis(2-acetoxyetl1yl)amides oi rapeseed acids IO-undecenolc acid 4-(io undecenoyD-rnorpholine.

Sorbic acid 4-sorboylmorpholinc Morphoiine P ipei'ri In a Piperazine hexhydrate.

a The new antimicrobial compounds are italicized.

b Good; +=Fair; =None; oo=Organisrn failed to grow on disc; o=Slight growth oi organism on disc.

e A=Eecherichia 6011 B=Micrococcus pyogenes; C=Candida albicam; D=Candida wer'necki; E=Epidermophyton floccoaum; F=Keratinomucas ajelloi; G=Microsporum cams; H=Microspormn cookii; I=Microsporum gypseum. J=Microsporu1n Nanum; K= Tricbophyton concentricum; L TrichopM/ton epz'lans.

Iodine value of 66.0; Thiocyanogcn value of 6513. Iodine value oi 58.7; Thiocyanogen value of 53.9. I Oxirane content of 2.78%. I Oxirane content of 2.35%. h Iodine value oi 45.3; Thiocyanogen value oi 41.5.

TAB LE IIB Antimicrobial activity b Compound I Micro-organisms G-hydroxycaproic acid 4-(G-hydroxycapro1 l)marpholine 4-(6-hydroxycaproyl)-2,6-dirnethylmorpholine Lactone of G-hydroxycaproic acid Ethyl 6-hydroxycaproatc Sodium 6hydroxycaproatc fi-aminocaproic acid Lactam of S-aminocaproic acid Nonanoic acid 4nonanoy1m0rpholine Decanoic acid 4decanoylmorpholinc 4-decan0yZ2,6-dimethylmorpholine N-butyldecanamidc 1,: u

N-cyclohezyldecanamide N,N-bis(Z-decanoylozyethyl)decamzmide N-decanoylpiperdine- N-decanoylhemmethylenimine Ndecanoyl-Nmethylpiperazine. N,N-didecanoylpiperazine N-decanoyltetrahydroguinoline Laurie acid 4-iauroylrnornholinfl N,N-bis(z-hydroxycthyl)laurumide. N,N-bis(Q-dccanoulozyethyl)lauram N,N-his (Z-lauroyloxyethyl)Zauramirie- Palrnitic acid 4-palrnitoylmorpholine Stearic acid 4-stearoylrnorpho 6(7)-hydroxystoaric a 4-60)-hz/drozustearoyl)morph 6,7-epoxystearic acid 12-hydroxystearic acid 4-(l2-hydroxystearoyl)mornhnline Methyl 12-hydroxystearate 4-(epoxystearoyl)morpholine N,N-bis(2-acetoxyethyl) epoxystearamid N,N-bis(2-aeetoxyethyl)dicpoxystearamide Oleic acid -oleoylrnorp line 4-uleo l-Z,G-dimethylmorpholim. 4-(epoxyoctadeccnoyl) morpholi Ricinoleic acid 4-ricinoleoylmorpholine Methyl ricino1eate 4-(12-propiono1yoleoyl)morpho N-tert-butuloleamld N ,N-bis(2hydroxyethy1) oleamide N,N-bis(Z-acetoxyethyl)olearnide N,N-bis(Z-benzoyloxyethyl)oleamlde N ,N-bis(2-aeetoxyethyl)epoxyoctadecenamide N-0leoylhezamethz a N-oZeoyl-Nmtethz lpiperazine Petroselinic acid- 1,-petroselinog/lmorpholim Methyl petroselinate Elaidic acid 4-elaidoylmornhnli Linoleic acid 4-linoleoylmorpholine Dimerlzed linoleic acid (Empol 1022) Dimerized 4-linoleoyimorpholine Partially hydrogenated cottonseed acids. Morpholides of partially hydrogenated cottonseed acids.

Morpholides of partially epoxidized cottonseed acids. N,N-bis(2-acetoxyetl1yl)amides of partially epoxidized cottonseed acids N,I Iihbis (2-acetoxyethyl)amides of partially hydrogenated cottonseed ac s.

Parsley seed acids Morpholides ojparsleu seed oil fatty acids Morpholides of rapeseed acids N,N-bis(2-acetoxyethyDamides oi rapeseed acids.- IO-undecenoic acid 4-(ldundecenoybmorpnlinp Sorbic acid 4-sorb oylmorpholine- Morpholine- Piperidine Piperazine hydrate llllllllllll lllAi The new antimicrobial compounds are italicized.

good; fair; none; 00 Organism failed to grow on disc. 0 slight growth of organism on disc.

M Trichophyton equinu'm; N Tricophyto'n. ferrugineum; O Trichophyton gallinae; P Trichophyton magm'm'; Q, Trichophyton mentagroph tea var. interdigitalea; R Trichophyion ,mentagrophytes var. granulare; S Trichophyton rubrum;'1 Trichophyton .sabouraudi,

U Trichophyton schoenleini; V Trichophyton chophyton tonsuram; X Trichophyton violaceum.

Iodine value of 66.0; Thiocyanogen value 01' 65.8. =Iodine value of 58.7; Thiooyanogen value of 53.9. Oxirane content of 2.78%. Oxirane content of 2.35%. Iodine value of 45.3; Thiocyanogen value of 41.5.

sulfurium; W Tri- Example 1 4-(6-lzydr0xycaproyl)m0rph0line.Eighty grams (0.7 mole) of the epsilon-lactone of 6-hydroxycaproic acid and 122 grams (1.4 moles) of morpholine were stirred and refluxed for 19 hours. Titration of one-milliliter aliquots periodically for unreacted morpholine indicated that 92% of the lactone had reacted with the morpholine. After the excess morpholine had been stripped under reduced pressure, the product was distilled under high Vacuum. The fraction distilling between 149151 C. at 2 microns was crystallized from ethyl acetate. Nitrogen content of the product, 4-(6-hydroxycaproyl)morpholine, was 6.88% (theory 6.96%).

Example 2 4-decan0yl-2,6 dimelhyli110rph0line.-Eight and sixtenths grams (0.075 mole) of 2,6 dimethylrnorpholine were dissolved in 20 milliliters of benzene. Then 7.08 grams (0.037 mole) of decanoyl chloride were added dropwise with stirring. After stirring for an additional hour, the reaction mixture was filtered, washed successively with dilute hydrochloric acid and water, and dried over anhydrous sodium sulfate. Free acid was removed by percolating the benzene solution through a column of activated alumina and eluting the morph-olide with a 1:1 ethanolbenzol mixture. The solvent was then removed by stripping under reduced pressure. Nitrogen content of the product, 4-decanoyl-2,6-dirnethylmorpholine, was 5.04% (theory 5.21%).

Example 3 N-baiyla'ecanamide.-N-butyldecanamide was prepared by the procedure of Example 2 from 6.44 grams (0.088 mole) of n-butylamine and 8.39 grams (0.044 mole) of decanoyl chloride. Nitrogen content of the product, N- butyldecanamide, after recrystallization three times in 95% methanol, was 6.09% (theory 6.16%).

Example 4 N-isoamyldecanamide.-N-isoamyldecanarnide was prepared by the procedure of Example 2 from 7.22 grams (0.083 mole) of isoamylamine and 7.90 grams (0.041 mole) of decanoyl chloride. The product, N isoa-myldecanamide, had a nitrogen content of 5.39% (theory 5.80%).

Example 5 N cyclolzexyldecanamide.N cyclohexyldecanamide was prepared by the procedure of Example 2 from 7.82 grams (0.079 mole) of cyclohexylamine and 7.53 grams (0.039 mole) of decanoyl chioride. The product, N-cyclohexyldecanamide, had a nitrogen content of 5.39% (theory 5.53

Example 6 N,N-bis(2 decanoyloxyethyl)decanamide.-A mixture of 5.0 grams (0.048 mole) of diethanolamine, 25.8 grams (0.150 mole) of decanoic acid, and 20 milliliters of henzone was refluxed in an apparatus equipped with a Dean- Stark trap until the evolution of water ceased. The reaction mixture was diluted with 200 milliliters of commercial hexane, washed successively with dilute hydrochloric acid and water, and dried over anhydrous sodium sulfate. Free acid was removed by percolating the hexane solution through a column of activated alumina, and eluting the amide with a 1:1 hexane-ethanol mixture. The solvent was removed by stripping under reduced pressure. The product, N,N-'bis(2 decanoyloxyethyl)decanamide, had a nitrogen content of 2.62% (theory 2.47%).

Example 7 N decanoylhexamethylenimine.-N decanoylhexamethylenimine was prepared by the procedure of Example 2 from 5.2 grams (0.053 mole) of hexamethylenimine, 10.0 grams (0.053 mole) of decanoyl chloride, and 4.2 grams (0.05 3 mole) of pyridine. The product, N-decanoylhexamethylenimine, had a nitrogen content of 5.31% (theory 5.53%).

Example 8 N-decanoyl-N'-methylpiperazine.-N decanoyl N- methylpiperazine was prepared by the procedure of Example 2 from 5.24 grams (0.052 mole) of N-methylpiperazine, 4.2 grams (0.053 mole) of pyridine, and 10.0 grams (0.053 mole) of decanoyl chloride. The product, N- decanoyl-N'-methylpiperazine, had a nitrogen content of 10.83% (theory 11.01%).

Example 9 N,N'-didecan0ylpiperazine.-A mixture of 13.8 grams (0.16 mole) of piperazine, 60.2 grams (0.35 mole) of decanoic acid, and 20 milliliters of benzene was refluxed in an apparatus equipped with a Dean-Stark trap for 12 hours. The reaction mixture was diluted with milliliters of commercial hexane, washed successively with dilute hydrochloric acid and water, and dried over anhydrous sodium sulfate. After stripping the solvent, the reaction products were dissolved in carbon tetrachloride. The unreacted decanoic acid was converted to its potassium salt with 4% alcoholic potassium hydroxide. Then the mixture was extracted twice with water to remove the potassium decanoate. The carbon tetrachloride solution was dried over anhydrous sodium sulfate. After stripping the solvent, the amide was crystallized from ethanol. The product, N,N-didecanoylpiperazine, had a nitrogen content of 7.07% (theory 7.09%).

Example 10 N-decanoyltetrahydr0quin0line.-N decanoyltetrahydroquinoline was prepared by the procedure of Example 2 from 7.0 grams (0.053 mole) of tetrahydroquinoline, 4.2 grams (0.053 mole) of pyridine, and 10.0 grams (0.053 mole) of decanoyl chloride. The product, N-decanoyltetrahydroquinoline had a nitrogen content of 4.79% (theory 4.87%

Example 11 N,N-bis(Z-hydrOxyethyZ)laaramide.-Fifty-eight grams (0.27 mole) of methyl laurate was added dropwise to a stirred mixture of 28.4 grams (0.27 mole) of diethanolamine and 1.0 gram (0.043 mole) of sodium (dissolved in a small amount of methanol) under reduced pressure (60 millimeters) at 65 C. Stirring and heating was continued for an additional hour, until the evolution of methanol was complete. After the catalyst was neutralized by adding 3.5 grams (0.046 mole) of glycolic acid, the reaction mixture was dissolved in commercial hexane and filtered. After washing with water, the solvent was removed under reduced pressure. The product N,N-bis(2-hydroxyethyl) lauramide, had a nitrogen content of 4.87% (theory 4.87%

Example 12 N,N-bz's (2 decanoyloxyethyl) laaramide.-Seven grams (0.037 mole) of decanoyl chloride was added dropwise to a stirred mixture of 5 .0 grams (0.017 mole) of N,N-bis(2- hydroxyethyl)lauramide, 3.0 grams (0.038 mole) of pyridine, and 20 milliliters of dioxane. Stirring was continued for an additional 40 minutes. After hydrolyzing the ex cess decanoyl chloride with water, 100 milliliters of hexane was added. The pyridine hydrochloride and dioxane were removed by repeated water washings. The hexane solution was dried over anhydrous sodium sulfate, filtered, passed through an activated alumina column to remove residual free acid, and disolventized under reduced pressure. The product, N,N-bis(Z-decanoyloxyethyl)lauramide had a nitrogen content of 2.41% (theory 2.34%)

Example 13 N,N bis(2 lauroyloxyethyl)lauramia'e.-N,N-bis(2 lauroyloxyethyl)lauramide was prepared by the procedure of Example 6 from 5.0 grams (0.048 mole) of diethanolamine and 30.0 grams 0.150 mole) of lauric acid. The product, N,N bis(2-lauroyloxyethyl)lauramide, had a nitrogen content of 2.22% (theory 2.15%)

1 1 Example 14 v4 (6(7) hydroxystearoyl) morpholine. Six grams (0.02 mole) of 6(7)-hydroxy-stearic acid, 10.5 grams (0.12 mole) of morpholine, and 10 milliliters of benzene Were stirred under reflux in an apparatus equipped with a Dean-Stark trap. Periodic infrared analysis indicated that the reaction had approached equilibrium after 60 hours. The reaction mixture was diluted with 50 milliliters of diethyl ether and washed three times with 40-milliliter portions of 1% sodium hydroxide solution (4:1 mixture of water and isopropanol). Then the ether-benzene solution was washed three times with 50-milliliter portions of water. After drying the ether-benzene solution over anhydrous calcium sulfate, the solvent was stripped. The product, 4-(6(7)-hydroxystearoyl)morpholine, was recrystallized from petroleum ether. Elementary analysis indicated 3.75% nitrogen (theory 3.79%

Example Example 16 4-(12-pr0pionoxy0le0yl)morpholine. Three hundred twelve grams (1 mole) of methyl ricinoleate and 174 grams (2 moles) of morpholine were stirred and refluxed in an apparatus equipped with a short Vigreaux column, reflux condenser, and Dean-Stark trap. Titration of onemilliliter aliquots periodically for unreacted morpholine indicated that the reaction had approached equilibrium after 36 hours. After the excess morpholine had been stripped under reduced pressure, the product was distilled under high vacuum. 4-ricinoleoylmorpholine distilled between 243246 C. at 0.2 millimeter. Elementary analysis indicated 3.80% nitrogen (theory 3.81%). One hundred fifty grams (0.41 mole) of 4-ricinoleoyl-morpholine and 150 grams (1.15 mole) of propionic anhydride were stirred and refluxed for three hours. After the propionic acid and excess propionic anhydride were distilled under reduced pressure, the product was distilled under high vacuum. The fraction distilling between 250-254" C. at 0.3 millimeter was dissolved in benzene and percolated through a column of activated alumina. The morpholide was eluted with a 1:1 ethanol-benzene mixture. After stripping the solvent under reduced pressure, the product, 4-(12-propionoxyoleoyl)morpholine, had a nitrogen content of 3.12% (theory 3.31%).

Example 17 N-tert-butyloleamide.-N-tert-butyloleamide was prepared by the procedure of Example 2 from 19.6 grams (0.27 mole) of tert-butylamine and 40.0 grams (0.13 mole) of oleoyl chloride. The product, N-tert-butyloleamide, had a nitrogen content of 4.19% (theory 4.12%).

Example 18 N-oleoylhexamethylenimine. N-oleoylhexamethylenimine was prepared by the procedure of Example 2 from 9.88 grams (0.10 mole) of hexamethylenimine, 8.0 grams (0.10 mole) of pyridine, and 30.0 grams (0.10 mole) of oleoyl chloride. The product, n-oleoylhexamethylenimine, had a nitrogen content of 3.79% (theory 3.85%).

Example 19 N 0le0yl-N'-metlzylpiperazine. N-oleoyl-N'-methylpiperazine was prepared by the procedure of Example 2 from 23.3 grams (0.23 mole) of N-methylpiperazine and 35.0 grams (0.12 mole) of oleoyl chloride. The product, N-oleoyl-N-methylpiperazine, had a nitrogen content of 7.44% (theory 7.68%).

Example 20 4-petroselin0ylmorpholine.--4-petroselinoylmorpholine was prepared by the procedure of Example 6 from 6.2 grams (0.071 mole) of morpholine and 10.0 grams (0.035 mole) of petroselinic acid. The product, 4-petroselinoylmorpholine, had a nitrogen content of 3.94% (theory 3.98%).

Example 21 Morpholides 0f parsley seed oil fatty acids.-The essential oil was removed from the crude hexane-extracted parsley seed oil by steam distillation. The parsley seed oil was saponified with potassium hydroxide, and the soaps were decomposed with hydrochloric acid to obtain the free parsley seed oil fatty acids.

The morpholides of parsley seed acids were prepared by the procedure of Example 6 from 32 grams of morpholine and 68 grams of parsley seed acids. The product, the morpholides of parsley seed oil fatty acids, had a nitrogen content of 3.43%.

We claim:

1. A process of controlling microbial activity comprising contacting the microbe with an antimicrobial composition containing as its essential active ingredient a member selected from the group consisting of 4-(6-hydroxycaproyl)morpholine, 4 decanoyl 2,6 dimethylmorpholine, 4 oleoyl 2,6 dimethylmorpholine, 4 petroselinoylmorpholine, and the morpholides of parsely seed oil fatty acids.

2. The process of claim 1 wherein the member is 4-(6- hydroxycaproyl morpholine.

3. The process of claim 1 wherein the member is 4- decanoyl-2,6-dimethylmorpl1oline.

4. The process of claim 1 wherein the member is 4- oleoyl-2,6-dimethylmorpholine.

5. The process of claim 1 wherein the member is 4- petroselinoylmorpholine.

6. The process of claim 1 wherein the member is morpholides of parsely seed oil fatty acids.

References Cited by the Examiner Dictonary of Organic Chemistry IV (1953), Oxford, University Press, New York, p. 78.

Novak et al.: The Journal of the American Oil Chemists Society, 38 (1961), 321-324.

Small: Chem. Abstracts, 43 (1949), 8573.

JULIAN S. LEVITT, Primary Examiner.

STANLEY I. FRIEDMAN, Assistant Examiner. 

1. A PROCESS OF CONTROLLING MICROBIAL ACTIVITY COMPRISING CONTACTING THE MICROBE WITH AN ANTIMICROBIAL COMPOSITION CONTAINING AS ITS ESSENTIAL ACTIVE INGREDIENT A MEMBER SELECTED FROM THE GROUP CONSISTING OF 4-(6-HYDROXYLCAPROYL, 4 - DECANOYL -2,6 - DIMETHYLMORPHOLINE, 4 - OLEOYL - 2,6 - DIMETHYLMORPHOLINE, 4 - PETROSELINOYLMORPHOLINE, AND THE MORPHOLIDES OF PARSELY SEED OIL FATTY ACIDS. 